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[摘要]
目的 建立同时测定六味地黄胶囊中毛蕊花糖苷、马钱苷、芍药苷和丹皮酚4种成分含量的高效液相色谱(HPLC)测定方法。方法 应用HPLC-梯度洗脱法,采用Kromasil C18(250 mm×4.6 mm,5 μm)色谱柱,以1%冰醋酸为流动相A,乙腈为流动相B进行梯度洗脱,体积流量为1.0 mL/min,检测波长为230 nm。结果 毛蕊花糖苷、马钱苷、丹皮酚和芍药苷测定浓度的线性范围分别为6~60、15~150、50~500 μg/mL和5~50 μg/mL,r值均大于0.999,平均加样回收率分别为98.3%、99.3%、99.0%和99.0%,相对标准偏差(RSD)值均小于0.9%(n=9)。结论 该实验方法简便、结果准确,可有效地用于六味地黄胶囊的质量控制。
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[Abstract]
Objective An HPLC method was established for determining four constituents (verbascoside, loganin, paeoniflorin, and paeonolum ) in Liuwei Dihuang Capsule. Methods HPLC-gradient elution method was applied. The column was Kromasil C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was 1% acetic acid solution (A) and acetonitrile (B). The detection wavelength was set at 245 nm. Results The linear range of density was 6—60 μg/mL for verbascoside, 15—150 μg/mL for loganin, 50—500 μg/mL for paeonolum, and 5—50 μg/mL for paeoniflorin, respectively. The correlation coefficient of all curves were better than 0.999. The average recoveries of four analytes were 98.3% for verbascoside, 99.3% for loganin, 99.0% for paeonolum, and 99.0% for paeoniflorin, and the RSD values were less than 0.9% (n = 9). Conclusion The developed HPLC method could be used for the quality control of Liuwei Dihuang Capsule.
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