目的 建立刺五加中异嗪皮啶的定量方法，并对主产区采集的刺五加野生药材的不同部位、不同生长年限及不同生长环境的异嗪皮啶的量进行测定，为分析刺五加野生药材的质量提供参考。方法 刺五加异嗪皮啶的提取采用超声提取法，HPLC法测定，色谱柱为Diamonsil C₁₈（250 mm×4.6 mm，5 mm），流动相为乙腈-0.1%磷酸（20：80），检测波长344 nm，体积流量1.0 mL/min。结果 异嗪皮啶进样量在16.8～252.0 ng呈现良好的线性关系（r=0.999 9），平均回收率为100.4%，RSD值为1.0%（n=6）。刺五加不同部位中，皮的异嗪皮啶含量最高，茎次之，根及根茎含量较低。不同生长年限中，5年生刺五加异嗪皮啶含量最高，3年生含量次之，2年以下含量最低。阳坡生长的异嗪皮啶含量高于阴坡生长的药材。总体分析异嗪皮啶的含量黑龙江高于吉林。结论 本方法可操作性强，简便快速，重复性好，回收率高，稳定可靠，可作为刺五加药材的质控方法。鉴于野生药材生长年限不一，分布不集中，与栽培的药材相比含量波动大，因此，在药品生产过程中，根据对中间体或成品中异嗪皮啶含量的要求，有必要建立刺五加药材异嗪皮啶的含量标准，以保证生产药品的质量。

Objective To establish a method for the determination of isofraxidin in Acanthopanax senticosus, and the content of isofraxidin in wild A. senticosus collected from main producing areas in different parts, growth years, and growth conditions were measured. This provides a reference for quality analysis of wild A. senticosus. Methods Isofraxidin in A. senticosus was extracted using ultrasonic extraction. The HPLC analysis was carried out on a Diamonsil C₁₈ column (1) (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.1% phosphoric acid (20:80) and the flow rate was 1.0 mL/min. The detective wavelength was set to 344 nm. Results The calibration curve was linear with the sample load of 0.016 8—0.252 0 μg (r = 0.999 9). The average recovery was 100.4% with RSD of 1.0% (n = 6). The highest content of isofraxidin from same herb was in skin, followed by stems, and in roots and rhizomes the content was the lowest. The highest content of different ages was for five years to birth, followed by three years, and for less than two years was the lowest. The growth content of herbs in sunny area was higer than that in shady area. Based on the above analyzed, the content of isofraxidin in A. senticosus from Heilongjiang were higher than those from Jilin. Conclusion Considering about the different growth periods, distribution and fluctuation of content compared with the cultivation of medicinal materials, it is necessary to establish the content standards for isofraxidin of A. senticosus to ensure the quality of pharmaceuticals production, according to the isofraxidin content requirements of the intermediate or finished medicine. This method is simple, rapid, accurate, and replicate. And it can be adopted for the quality control of A. senticosus.

天津市重大科技专项（工程）项目：龙加通络胶囊产业化研究