[关键词]
[摘要]
目的 制备新型抗炎镇痛化合物4-邻甲苯磺酰氧基苯并噁唑酮(MBB)脂质体并对其进行质量控制方法研究。方法 采用薄膜分散法制备MBB脂质体,观察脂质体的形态,测定其粒径与电位,采用HPLC法测定其含量,并考察其包封率、渗漏率、稳定性等。结果 制备的MBB脂质体平均粒径为46.3 nm,电位为-60.25 mV,包封率为94%,且于4 ℃下储存较稳定。结论 所制备的脂质体粒径均一,包封率高,稳定性好,所建立的HPLC分析方法准确可靠、重复性好、操作简便、专属性强,符合质量测定要求。
[Key word]
[Abstract]
Objective To prepare the liposomes of 4-O-methyl-benzenesulfonyl-2 (3H)-benzoxazolone (MBB), an anti-inflammatory agent, and to investigate the quality control methods. Methods The liposomes were prepared by the film dispersion method, then the morphology and diameter were detected. The content of MBB was determined by HPLC method, and the entrapment efficiency, percolation rate, and stability of liposomes were investigated, respectively. Results The mean diameter of the liposomes was 46.3 nm, and the potential was -60.25 mV. The encapsulation efficiency was 94%. The liposomes were stable at 4 ℃. Conclusion The prepared MBB liposomes show homogeneous diameter, high encapsulation efficiency, and good stability. The HPLC method is sensitive and accurate with good specificity which could be used for the quality control of MBB liposomes.
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[基金项目]
山西省科技攻关项目(20110321076)