[关键词]
[摘要]
目的 评价12种降压西药的寒热药性。方法 用噻唑蓝(MTT)比色法检测12种降压西药对HepG2细胞体外增殖的影响,并用倒置显微镜观察其对细胞形态特征的影响。结果 利血平、倍他洛克、卡托普利、硝苯地平、氨氯地平的药性为寒性;普萘洛尔、培哚普利、雷米普利、氯沙坦钾、尼群地平、吲达帕胺、氢氯噻嗪为热性。形态学观察表明利血平、倍他洛克、卡托普利、硝苯地平、氨氯地平使细胞密度减小,固缩变圆;普萘洛尔、培哚普利、雷米普利、氯沙坦钾、尼群地平、吲达帕胺、氢氯噻嗪在低浓度时可使细胞密度增加,结构致密、生长旺盛。结论 细胞学方法能够用于西药寒热药性的评价,这为西药中药化提供了一种简单实用的评价寒热药性的方法。
[Key word]
[Abstract]
Objective To evaluate the cold-heat characteristics of 12 kinds of hypotensive drugs. Methods MTT assay was used to investigate the effect of the 12 kinds of hypotensive drugs on the growth and proliferation of cultured HepG2 cells in vitro. Morphological changes were observed through inverted microscope. Results Reserpine, Metoprolol, Captopril, Nifedipine, and AmLodipine showed cold or cool characteristics, while Propranolol, Perindopril, Ramipril, Losartan potassium, Nitrendipine, Indapamide, and Hydrochlorohthiazide showed hot or warm characteristics. Morphological observation demonstrated that the cell density of the cells treated with Reserpine, Metoprolol, Captopril, Nifedipine, and AmLodipine decreased, but the cell density of the cells treated with Propranolol, Perindopril, Ramipril, Losartan potassium, Nitrendipine, Indapamide, and Hydrochlorothiazide increased. Conclusion The cytological method could be used to evaluate the cold-heat characteristics of Western medicine. This simple and reliable method will promote the Chinese medicalization of Western medicine.
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[基金项目]
国家自然科学基金资助项目(20872118,30070905);教育部博士点基金资助项目(20070697012);陕西省重大科技专项资助项目 (2008ZDKG-67);陕西省重点实验室基金资助项目(08JZ74,2010JS097,11JS090)