[关键词]
[摘要]
目的 探讨延龄草苷(trillin)对小胶质细胞(BV-2)的炎性激活的抑制作用。方法 不同浓度的延龄草苷孵育0.5 h,然后用脂多糖进行刺激,培养24 h后收集上清,Greiss法检测NO浓度;CCK-8检测延龄草苷对小胶质细胞活力的影响;Elisa检测肿瘤坏死因子(TNF-α)的浓度;实时定量PCR(RT-PCR)检测TNF-α、IL-1β、IL-6、iNOS、COX-1、COX-2基因的表达。结果 在不影响细胞活力的前提下,延龄草苷能明显抑制NO的释放,同时能抑制激活诱导细胞死亡,能明显抑制TNF-α的产生,能明显抑制TNF-α、IL-1β、IL-6、iNOS、COX-2 mRNA的表达,但是不能抑制COX-1 mRNA的表达。结论 延龄草苷可以抑制LPS诱导的小胶质细胞的炎性激活,抑制炎性基因的表达。
[Key word]
[Abstract]
Objective To study the inhibition of trillin on the inflammatory activation of microglia (BV-2). Methods The BV-2 cells were incubated with trillin at different concentration for 0.5 h and stimulated with lipopolysaccharide (LPS). The supernatant was collected after 24 h culture, and the NO level was detected by Greiss method; The activity of BV-2 cells was detected with CCK-8; The expression of tumor necrosis factor (TNF-α), IL-1β, IL-6, iNOS, COX-1, and COX-2 was detected by real time quantitative (RT-PCR). Results Trillin can inhibit the NO release in microglia induced by LPS without affecting the cell viability and inhibit the inflammatory factors such as TNF-α, IL-1β, IL-6, iNOS, and COX-2. Conclusion Trillin can inhibit the inflammatory activationof BV-2 cells induced by LPS and inhibit the expression of inflammatory factor.
[中图分类号]
[基金项目]
国家重大新药创制项目(2011ZX09201-201)