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[摘要]
目的 研究平炎舒消膏(PSO)的抗炎作用并初步探讨其作用机制。方法 采用小鼠腹腔毛细血管通透性模型、大鼠足肿胀模型及大鼠棉球肉芽肿模型,观察PSO的在体抗炎作用。采用酶联免疫吸附法(ELISA)和生物测定法,观察PSO对脂多糖(LPS)诱导的巨噬细胞(RAW264.7)释放的白细胞介素-1(IL-1β)和肿瘤坏死因子-α(TNF-α)含量及生物学活性的影响,探索PSO的抗炎作用机制。结果 体内实验中,PSO显著抑制醋酸所致小鼠腹腔毛细血管通透性升高,明显降低大鼠自身血所致足跖肿胀,减轻大鼠棉球肉芽肿。体外实验中,PSO浓度相关性地抑制LPS诱导的RAW264.7释放IL-1β及TNF-α,降低IL-1β对胸腺细胞的增殖效应及TNF-α对L-929细胞的杀伤作用。结论 平炎舒消膏可减轻各期炎症形成,其机制可能与抑制巨噬细胞释放炎性因子有关。
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[Abstract]
Objective To study the anti-inflammatory effects of Pingyan Shuxiao Ointment (PSO) and its possible mechanism. Methods The anti-inflammatory effects were investigated in the peritoneal capillary permeability model of mice induced by acetic acid, in the paw edema model of rats induced by autologous blood, and granuloma model of rats induced by cotton ball. The anti-inflammatory mechanism of PSO was observed by assessing the content and biological activity of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) on RAW264.7 cells induced by lipopolysaccharide (LPS), using enzyme-linked immunosorbent (ELISA) and biological assay. Results PSO in vivo significantly improved the degree of the peritoneal capillary permeability of mice, decreased autologous blood-induced paw edema, and reduced cotton ball-induced granuloma of rats. PSO in vitro concentration-dependently inhibited LPS-induced RAW264.7 cell releasing IL-1β and TNF-α, reduced IL-1β’s proliferation effect on thymocyte and TNF-α’s capability on killing L929 cell. Conclusion PSO has the obvious anti-inflammatory effect, which may be mediated through the inhibition of the cytokines levels in the inflammatory tissues.
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