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[摘要]
目的 建立消毒饮优化方胶囊中异嗪皮啶的HPLC测定方法。方法 采用phenomenex Synergi 4 u Fusion-RP 80A柱(250 mm×4.60 mm,4 μm),流动相为乙腈-1%磷酸(20∶80),体积流量为1.0 mL/min,柱温为40 ℃,检测波长为342 nm。结果 异嗪皮啶进样量在0.100 0~1.000 0 μg与峰面积呈良好的线性关系(r=0.999 9),平均回收率为95.04%,RSD=1.84%(n=6)。结论 该方法操作简便、准确、专属性强、重复性好,可用于消毒饮优化方胶囊中异嗪皮啶的含量测定。
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[Abstract]
Objective To establish a method for the content determination of isofraxidin in Optimizing Xiaoduyin Capsules. Methods The HPLC was performed on the phenomenex Synergi 4 u Fusion-RP 80A column (250 mm × 4.60 mm, 4 μm) with the mobile phase of acetonitrile-1% phosphoric acid (20:80); The flow rate was 1.0 mL/min, the column temperature was 40 ℃, and the detection wavelength 342 nm. Results The sample size of isofraxidin during the range of 0.100 0—1.000 0 μg showed a good linear relation with the peak area (r = 0.999 9), and the average recovery rate was 95.04%, with RSD of 1.84% (n = 6). Conclusion The method is simple, reliable, and specific, with good reproducibility, and could be used to determine the content of isofraxid in Optimizing Xiaoduyin Capsules.
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[基金项目]
广西科学研究与技术开发计划课题(桂科攻11107009-2-4);广西壮族自治区中医药科技专项课题(GZYZ1125);广西教育厅校地校企共建科技创新平台项目(桂教科研 [2011] 9号);广西教育厅校地校企共建科技创新平台项目(桂教科研 [2013] 8号-3)