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[摘要]
目的 观察、追踪毛冬青抑制口腔常见致龋菌(变形链球菌和具核梭杆菌)的有效部位和成分。方法 通过系统溶剂萃取、大孔树脂及硅胶柱色谱分离等方法以及质谱和核磁共振等鉴定技术,结合液体二倍稀释法,研究毛冬青不同部位及单体对变形链球菌和具核梭杆菌的最低抑菌浓度(MIC),1%玉洁纯作为阳性对照药。结果 毛冬青三萜总皂苷和冬青素对两种主要口腔致病菌均具有一定的抑制作用,特别对变形链球菌具有显著地抑制效果。结论 毛冬青三萜总皂苷和冬青素对变形链球菌具有很强的抑制作用,具有开发成为抗龋齿的保健牙膏或药品的潜力。
[Key word]
[Abstract]
Objective To study and explore the inhibitory effects of the active fractions and compounds from Ilex pubescens on the growth of two major oral pathogens Fusobacterium nucleatum (F.n.) and Streptococcus mutans (S.m.) in vitro. Methods The I. pubescens extract was prepared by organic solvent method and then was separated by macroporous resin and silica gel column chromatograph methods. Sequently, the compounds were identified by the mass spectromety (MS) and nuclear magnetic resonance (NMR) methods. Finally, F.n. and S.m. were chosen as the experimental bacterial strains. The liquid media two-fold dilution method was used to study the minimum inhibitory concentration (MIC) of active fractions and compounds from I. pubescens with 1% triclosan as the positive control. Results Both the total triterpenoid saponins and ilexgenin A showed the effects on the two oral pathogens for some extent, and especially had significant inhibitory activity against S.m. Conclusion The total triterpenoid saponins and ilexgenin A show the significant inhibitory effect on S.m., and may develop into the toothpastes or drugs with anti-caries function.
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[基金项目]
江苏省自然科学青年基金(BK2012378);江苏省普通高校研究生科研创新计划项目(CX09B_284Z)