目的 研究蒽贝素对雄性大鼠血浆性腺激素水平、附睾精子质量、睾丸组织结构的影响，探讨蒽贝素降低雄性大鼠生育能力的作用机制。方法 给雄性性成熟大鼠sc蒽贝素铵盐溶液30 d，末次给药后2 h，测定血浆性激素水平；检查附睾精子质量；观察睾丸组织结构。结果 与对照组比较，给药30 d后，蒽贝素高、中剂量（40、20 mg/kg）组大鼠血浆睾酮、黄体生成素、卵泡刺激素水平、附睾精子活率、精子密度明显降低，组间差异有统计学意义（P＜0.05），附睾精子畸形率和顶体完整率无明显变化，显微镜下见睾丸组织呈萎缩性病变，曲精细管扁平、塌陷，管内精母细胞减少，附睾精细管内精子稀少。结论 蒽贝素抑制丘脑-垂体-性腺轴活动，降低雄性大鼠血浆性激素水平，引起睾丸萎缩和附睾精子活率、精子密度降低，使雄性大鼠生育能力下降。

Objective To study the effect of embelin on plasma sex hormone levels, epididymis sperm quality, testicular tissue structure, and its mechanism of reducing fertility action in male rat. Methods Sexually-mature rat received the hypodermic injection of embelin salt of ammonia, once daily, for 30 d. Plasma sex hormone levels were determined, epididymis sperm quality was checked up, and testicular tissue structure was observed 2 h after the last hypodermic injection of embelin. Results After hypodermic injection of embelin for 30 d, rat plasma levels of testosterone, luteinizing hormone, follicle-stimulating hormone, survival sperm rate, and sperm density in epididymis were decreased significantly, compared with control group (P < 0.05), no changes in sperm deformity rate and acrosome intact rate were found in all embelin-treated groups; convoluted seminiferous tubules were flat, collapsed, or atrophic pathological changes, decreased spermatogonium was observed in convoluted seminiferous tubule of testicular tissue, extremely low sperm counts were observed too in seminiferous tubule of epididymis under the microscope in high- (40 mg/kg) and mid-(20 mg/kg) dose embelin-treated groups. Conclusion Embelin inhibits the activity of hypothalamus-pituitary-sex gland axis, decreases plasma sex hormone level, leads to atrophy in testicular tissue, and decreases survival sperm rate and sperm density in epididymis, resulting in decreased fertility in male rats.

广西自然科学基金项目（2013GXNSFAA019261）；广西医疗卫生重点科研课题（重2012057）；广西中药质量标准研究重点实验室主任基金（桂中重科201103）