目的 建立测定通乐颗粒中2, 3, 5, 4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的方法。方法 采用HPLC法，色谱柱为Phenomenex C₁₈柱，检测波长为320 nm，流动相为乙腈-水（20∶80），体积流量为1.0 mL/min。结果 2, 3, 5, 4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在10～100 μg/mL与峰面积呈良好线性关系（r＝0.999 7），平均回收率为99.74%。结论 本方法准确、简便、重复性好，可用于通乐颗粒中2, 3, 5, 4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量测定。

Objective To establish an HPLC method for the determination of 2, 3, 5, 4′-tetetrahydroxy-stilbene-2-O-β-D-glucoside in Tongle Granule. Methods The content of 2, 3, 5, 4′-tetetrahydroxy-stilbene-2-O-β-D-glucoside was detected by HPLC. The separation was performed on a Phenomenex C₁₈ column with UV detection at 320 nm, the mobile phase consisted of acetonitrile-water (20 : 80), and the flow rate was 1.0 mL/min. Results The calibration curve of 2, 3, 5, 4′-tetetrahydroxy-stilbene-2-O-β-D-glucoside was linear within the range of 10—100 μg/mL (r = 0.999 7), and the average recovery was 99.74%. Conclusion The method to determine the content of 2, 3, 5, 4′-tetetrahydroxy-stilbene-2-O-β-D-glucoside is accurate, simple, and reproducible, and could be used in the quantitative analysis of 2, 3, 5, 4′-tetetrahydroxy-stilbene-2-O-β-D-glucoside in Tongle Granule.