[关键词]
[摘要]
目的 建立HPLC法测定忍冬藤中4种环烯醚萜苷的方法。方法 使用Diamonsil C18色谱柱(250 mm×4.6 mm,5 μm),乙腈(A)-0.05%磷酸水(B)梯度洗脱,0~10 min,10%~14% A;10~35 min,14% A;体积流量1.0 mL/min,检测波长236 nm。柱温30 ℃。结果 裂环马钱子苷酸、马钱苷、当药苷、secologanoside-7-methyl ester分别在0.806 4~32.256 μg、2.136~85.44 μg、0.793 6~31.744 μg、0.782 4~31.296 μg呈良好线性关系;回收率分别为101.27%、98.50%、101.34%、101.36%,RSD分别为2.30%、1.54%、2.10%、1.87%;结论 所建立的方法简便、准确、重复性好,可用于同时测定忍冬藤中4种环烯醚萜苷的量。
[Key word]
[Abstract]
Objective To establish an HPLC method for the determination of four iridoid glycosides in Lonicera Japonicae Caulis . Methods The Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile (A)-0.05% phosphoric acid (B) gradient elution: 0—10 min, 10%—14% A; 10—35 min, 14% A; flow rate of 1.0 mL/min, detection wavelength at 236 nm, column temperature of 30 ℃. Results The secologanic acid, loganin, sweroside, and secologanoside-7-methyl ester presented the good linear relationship in 0.806 4—32.256, 2.136—85.44, 0.793 6—31.744, and 0.782 4—31.296 μg, respectively. Recoveries were 101.27%, 98.50%, 101.34%, and 101.36% with RSD values of 2.30%, 1.54%, 2.10%, and 1.87%. Conclusion The established method is simple, accurate, and reproducible and could be used for the simultaneous determination of four iridoid glycosides in Lonicera Japonicae Caulis.
[中图分类号]
[基金项目]
天津市自然科学基金(11zcdzzc21000)