[关键词]
[摘要]
目的 观察养肝益水颗粒对自发性高血压大鼠肾组织转化生长因子-β1(TGF-β1)、磷脂酰肌醇-3激酶(PI3K)和蛋白激酶B(PKB)的影响,探讨养肝益水颗粒对原发性高血压早期肾损害的作用及机制。方法 取50只12周龄收缩压大于150 mmHg的自发性高血压大鼠,随机分为模型组,养肝益水颗粒高、中、低剂量组(10.8、5.4、2.7 g/kg)和阳性对照药厄贝沙坦组(0.015 g/kg),每组10只;另取10只Wistar雄性大鼠作为正常对照组,模型组和正常对照组ig给予等量蒸馏水,每天1次,共12周。给药前和给药12周后用酶联免疫吸附法(ELISA)检测各组大鼠尿微量白蛋白(UMA)浓度;末次给药后,用免疫组化法检测各组大鼠肾脏组织中TGF-β1和PI3K的表达;用ELISA法检测PKB的浓度。结果 养肝益水颗粒有效地降低了UMA浓度和肾脏组织TGF-β1;升高了肾脏组织PI3K和PKB水平。结论 养肝益水颗粒对高血压的早期肾损害有一定的抑制作用,其作用机制可能与影响TGF-β1、PI3K和PKB的含量有关。
[Key word]
[Abstract]
Objective To observe the effects of Yanggan Yishui Granule (YYG) on transforming growth factor-β1 (TGF-β1), phosphatidylinositol-3-kinase (PI3K), and protein kinase B (PKB) in spontaneously hypertensive rats (SHR), and to explore the underlying mechanisms of YYG in preventing and treating early renal damage. Methods Fifty 12 weeks-old male SHR with the systolic blood pressure > 150 mmHg were randomly divided into the model group, the high-, mid-, and low-dose (10.8, 5.4, and 2.7 g/kg) YYG groups, and the positive control (Irbesartan 15 mg/kg ) group, 10 SHR in each group. Another ten Wistar rats were included as the control group. Rats in model and control groups were given the same volume of distilled water once daily for 12 weeks. At pre-administration and after successive administration for 12 weeks, urine microalbumin (UMA) concentration was measured by ELISA; after the last administration, the levels of TGF-β1 and PI3K in liver tissue were determined by immunohistochemistry and PKB was detected by ELISA. Results YYG decreased UMA concentration and TGF-β1 in kidney, and increased PI3K and PKB levels in kidney. Conclusion YYG inhibites the early renal damage of SHR which might be related to its regulation on TGF-β1, PI3K, and PKB contents.
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[基金项目]
康缘中医药科技创新基金项目(2010HZ020)