[关键词]
[摘要]
目的 建立毛支清口服液中tortoside A的定量测定方法。方法 采用高效液相色谱(HPLC)法,选用Venusil MP C18分析柱(250 mm×4.6 mm,5 μm),乙腈-水(18∶82)为流动相,检测波长为207 nm,体积流量为1.0 mL/min,柱温30 ℃。结果 Tortoside A进样质量浓度为1.76~35.20 μg/mL与峰面积呈良好的线性关系,回归方程Y=83 335X-47 442,r=0.999 7,平均回收率为101.53%,RSD值为2.50%。结论 所建立的HPLC法简便、准确、灵敏度高、重复性好,可控制毛支清口服液的质量。
[Key word]
[Abstract]
Objective To establish a method for the determination of tortoside A in Maozhiqing Oral Liquid (MOL). Methods The tortoside A was determined by high performance liquid chromatography (HPLC). The Venusil MP C18 column (4.6 mm × 250 mm, 5 μm) was used, the mobile phase was acetonitrile-water (18:82), the detection wavelength was 207 nm, the flow rate was 1.0 mL/min, and the column temperature was 30 ℃. Results Tortoside A has a good linear relationship with the peak area within the range of 1.76—88.00 μg/mL with an regression equation Y = 83 335X-47 442 (r = 0.999 7). The average recovery was 101.53%, and RSD was 2.50%. Conclusion The established method of HPLC could be used for the quality control of MOL for its simplicity, accuracy, high sensitivity, and good reproducibility.
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[基金项目]
国家自然科学基金资助项目(81073151)