[关键词]
[摘要]
目的 提高完善祛风舒筋丸的质量标准。方法 采用薄层色谱法对处方中甘草进行了薄层色谱鉴别,采用高效液相色谱法对处方中秦艽的有效成分龙胆苦苷进行了测定。结果 薄层斑点清晰,分离度好,阴性无干扰;龙胆苦苷进样量在0.103 9~1.039 0 μg线性关系良好(r=0.999 0),平均加样回收率为95.92%,RSD为2.50%(n=6)。结论 定性、定量方法简便、准确、专属性强、能有效控制祛风舒筋丸的质量。
[Key word]
[Abstract]
Objective To revise and improve the quality standard of Qufeng Shujin Pill (QSP). Methods The quantity of glycyrrhetinic acid in Glycyrrhizae Radix et Rhizoma was identified by TLC and the determination of the gentiopicroside was conducted by HPLC. Results There were clear spots, good degree of separation, and no negative interference in the TLC identification. The linear range of calibration curve was 0.103 9—1.039 0 μg (r = 0.999 0). The average recovery rate was 95.92% with RSD of 2.50% (n = 6). Conclusion The method is accurate, sensitive, and reliable, and could be used in the quality control for QSP.
[中图分类号]
[基金项目]
《中国药典》2010版一部标准研究课题任务书(ZH-446)