目的 以黄连为对象探讨中药谱效关系研究新方法。方法 建立优化基于ATP生物发光的微量抑菌检测方法，与微量稀释法抑菌结果进行相关性考察。以所建立的抑菌检测方法检测黄连抑菌活性，整合黄连的化学与抑菌活性图谱，探讨黄连化学组分与活性之间的关系。结果 所建立的基于ATP生物发光抑菌检测法与微量稀释法相关性良好，分别检测大肠杆菌和金黄色葡萄球菌所得相关系数r值分别为0.998和0.957。黄连谱效相关研究结果显示，黄连中除主要有效成分黄连素外，还可能含有微量高效抑菌成分。结论 基于ATP生物发光的抑菌活性检测方法适用于中药抑菌活性的谱效关系研究，为中药质量控制和药效评价提供新的技术手段。

Objective To explore the new methods for the research of spectrum-effect relationship of Chinese materia medica (CMM), taking Coptidis Rhizoma as an object. Methods The trace bacteriostatic detection method based on ATP bioluminescence was established and optimized, and the correlation of the results between ATP bioluminescence and microdilution method was investigated. The antibacterial activity of Coptidis Rhizoma was detected by the antibacterial detection method. and the chemical and activity profiles of Coptidis Rhizoma were integrated to discuss the relationship between the chemical composition and the activities. Results The results obtained by ATP bioluminescence assay correlated well with those by microdilution method, the r values of Escherichia coli and Staphylococcus aureus were 0.998 and 0.957, respectively. The integration activity spectra showed that except berberine there may be other substances with the high antibacterial activity in Coptidis Rhizoma. Conclusion ATP bioluminescence could be used in the study on the spectrum-effect relationship of antibacterial activity in CMM, in order to provide a new method for the quality control and effect evaluation of CMM.

国家自然科学基金资助项目（81173471）；江苏高校优势学科建设工程项目资助