[关键词]
[摘要]
目的 建立短筒兔耳草中松果菊苷的RP-HPLC测定方法。方法 短筒兔耳草经流动相超声提取后,用RP-HPLC法测定。采用Alltech Prevail C18(250 mm×4.6 mm,5 μm)色谱柱,流动相为乙睛-甲醇-1%醋酸(10∶15∶75),体积流量1.0 mL/min,检测波长334 nm,柱温为室温。结果 松果菊苷进样量在0.46~18.40 μg内与峰面积呈良好的线性关系(r=0.999 9);平均回收率为101.11%,RSD为2.09%(n=6)。结论 采用RP-HPLC测定短筒兔耳草药材中松果菊苷的含量,该方法准确、快速、简便,重复性好,适用于短筒兔耳草中松果菊苷的质量控制。
[Key word]
[Abstract]
Objective To develop a RP-HPLC method for the determination of echinacoside in Lagotis brevituba. Methods L. brevituba was extracted with the mobile phase by ultrasound extraction and then determined by RP-HPLC. The chromatograph was carried out on Alltech Prevail C18 column (250 mm × 4.6 mm, 5 μm) with acetonitrile-methanol-1% acetic acid (10:15:75) as the mobile phase at the flow rate of 1.0 mL/min, and the detection wavelength was set at 334 nm at room temperature. Results The linear range of echinacoside was 0.46—18.40 μg (r=0.999 9). The average recovery of echinacoside was 101.11% and RSD was 2.09% (n=6). Conclusion The method is accurate, quick, simple, and reproducible, and it could be used for the quality control of L. brevituba.
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[基金项目]
《中国药典》2010版一部标准研究项目(YS-204)