[关键词]
[摘要]
目的 研究杜鹃素在正常大鼠体内的药动学特征。方法 杜鹃素单剂量ig给予大鼠后,采用HPLC法测定给药后不同时间点大鼠血浆中的杜鹃素,通过DAS软件程序模拟计算,得出杜鹃素在大鼠体内相应的药动学参数。结果 杜鹃素在大鼠体内的药动学模型符合二室模型,主要药动学参数为:t1/2α=(0.33±0.10)h,t1/2β=(15.22±8.98)h,CL/F=(14.89±3.45)L/(h?kg),Cmax=(1.61±0.14)mg/L,Tmax=(0.25±0.01)h,MRT(0-t)=(2.35±0.08)h,AUC(0-t)=(3.06±0.16)mg?h/L。结论 本研究建立的HPLC方法专属性强,简便、准确,可用于杜鹃素在大鼠体内的药动学研究;大鼠ig给予杜鹃素后,其在血浆中分布较快,半衰期较短。
[Key word]
[Abstract]
Objective To study the in vivo pharmacokinetic characteristics of farrerol in normal rats. Methods The plasma concentration of farrerol in rats after ig single-dose administration was determined by HPLC and the pharmacokinetic parameters were calculated by DAS software. Results The plasma concentration-time curve of farrerol was fitted to two-compartment model, and the main pharmacokinetic parameters were as follows: t1/2α of (0.33 ± 0.10) h, t1/2β of (15.22 ± 8.98) h, CL/F of (14.89 ± 3.45) L/(h?kg), Cmax of (1.61 ± 0.14) mg/L, Tmax of (0.25 ± 0.01) h, MRT(0-t) of (2.35 ± 0.08) h, and AUC(0-t) of (3.06 ± 0.16) mg·h/L. Conclusion The established HPLC method is specific, simple, and accurate, and could be used to study the pharmacokinetics of farrerol in rats. Rapid distribution and short half-life of farrerol in rat plasma are observed after ig administration.
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[基金项目]
国家自然科学基金资助项目(81172938)