[关键词]
[摘要]
目的 研究竹黄菌的液体发酵最优条件及生物学活性。方法 利用单因素实验方法比较不同培养条件对竹黄菌生长和产竹红菌素的影响,考察了竹黄菌发酵液抗菌和抗氧化的生物学活性。结果 竹黄菌的较优培养条件为:蔗糖2.0%,葡萄糖3.0%,土豆20%,pH 7.0,菌种活化后放置72 h接种;竹黄菌发酵液对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、酿酒酵母、黑曲霉和黑根霉等均表现出了较强的抗性,对氧自由基和·OH的清除率分别为84.2%和76.32%。结论 竹黄菌的液体发酵条件简单,发酵产物具有一定的广谱抗菌性能和抗氧化活性,在养殖业中有着良好的应用前景。
[Key word]
[Abstract]
Objective To investigate the optimal conditions for liquid fermentation of Shiraia bambusicola and its bio-activities. Methods With the method of single-factor-test, the fermentation conditions of S. bambusicola and the production of hypocrellin were optimized, and the antibiosis and anti-oxidative activities were investigated further. Results The results showed that the optimized culture conditions were as follows: sugar 2.0%, glucose 3.0%, potato 20%, initial pH 7.0, and the spawn was inoculated after being preserved for 72 h. The krausen of S. bambusicola was resistant to Escherichia coli, Bacillus subtilis, Staphyloccocus aureus, Saccharomyces cerevisiae, Aspergullus niger, and Rhizopus niger, and to the oxygen radicals and ?OH with the clearance rate of 84.2% and 76.32%, respectively. Conclusion The strain of S. bambusicola is cultivated with simple conditions. The krausen is a potential additive in the cultivation with excellent anti-oxidative activities and broad antibiosis.
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[基金项目]
义乌市科研计划项目(10-3-03)