[关键词]
[摘要]
目的 观察大黄素对人胃癌MNK-45和MGC8-03细胞增殖的影响。方法 采用MTT法测定MNK-45细胞和MGC8-03细胞增殖抑制率;采用AO/EB双染观察细胞形态学变化;采用流式细胞仪测定细胞周期。结果 大黄素(5~80 μg/mL)对MNK-45和MGC8-03细胞增殖均具有显著抑制作用。P<0.01或0.05),且药物剂量越大,作用时间越长,其抑制作用越强(P<0.01);大黄素作用于两种细胞后荧光显微镜下观察均显示细胞凋亡特征;大黄素使MNK-45和MGC8-03细胞增殖被阻滞于G0/G1期。结论 大黄素能抑制人胃癌MNK-45和MGC8-03细胞增殖,诱导其凋亡,影响其细胞周期时相的分布。
[Key word]
[Abstract]
Objective To observe the effects of emodin on the proliferation of human gastric carcinoma cell lines MNK-45 and MGC8-03. Methods MTT assay was used to measure the inhibitory rate on the cell growth of MNK-45 and MGC8-03. The morphological changes were determined using AO/EB staining. The changes of cell cycle were detected by flow cytometry. Results Emodin (5―80 μg/mL) had the significant inhibition on cell proliferation of MNK-45 and MGC8-03 (P < 0.01 or 0.05). In addition, the higher the dosage was, the longer the effective time was, and the stronger the inhibiton was (P < 0.01); After emodin treatment, the two cell lines showed the features of apoptosis; Emodin could make the cell proliferation of MNK-45 and MGC8-03 stay in the G0/G1 phase. Conclusion Emodin could inhibit the cell proliferation of human gastric carcinoma MNK-45 and MGC-803, induce the apoptosis of MNK-45 and MGC-803, and affect the distribution of cell cycle phase.
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[基金项目]
国家自然科学基金面上项目(81173171)