目的 研究10种常用平喘中药桑白皮、枇杷叶、百部、马兜铃、紫菀、款冬花、苦杏仁、苏子、葶苈子、银杏叶提取物对磷酸二酯酶4（PDE4）的抑制作用。方法 分别采用水煎煮、95%乙醇回流提取法提取药材，采用放射性³H标记液体闪烁计数法检测提取物对靶标PDE4的抑制活性。结果 10种平喘中药的水提物、95%乙醇提取物均显示一定的抑制PDE4活性。总体而言，平喘中药95%乙醇提取物对PDE4抑制活性高于水提取物。相同浓度下，桑白皮、枇杷叶、款冬花、马兜铃活性较高。结论 平喘类中药对PDE4靶标具有一定的抑制作用，可能与其平喘作用相关。

Objective To study the inhibition of ten common anti-asthma traditional Chinese medicines (TCMs) towards phosphodiesterase 4 (PDE4). Methods The TCMs were extracted with boiling water and 95% ethanol reflux, respectively. The inhibition against PDE4 was carried out by radioactive ³H liquid scintillation counting method. Results Both water extracts and ethanol extracts exhibited obvious inhibition against PDE4. In general, the ethanol extracts of ten TCMs showed more potent activities than those of water extracts. At the same concentration, Mori Cortex, Eriobotryae Folium, Farfarae Flos, and Aristolochiae Fructus exhibited obvious activities. Conclusion The common anti-asthma TCMs exhibit obvious inhibition towards PDE4, which may be relative to their treatment of relieving asthma.

国家自然科学基金资助项目（21103234）；教育部留学回国人员启动基金（2008[290]）；广东自然科学基金团队项目（S2011030003190）；中山大学青年教师重点培育计划（11ykzd05）