目的 建立紫外分光光度法测定枸杞多酚提取物中的总多酚。方法 采用紫外分光光度法测定枸杞多酚提取物中总多酚，用水稀释样品，加入显色剂Filin-Ciocalteu试剂，在760 nm处测定吸光度，用同样方法测定并绘制标准曲线。结果 紫外分光光度法测定枸杞多酚提取物中总多酚的量分别为24.03%、26.38%、25.45%、28.27%、29.08%，线性范围1～8 μg/mL，R²＝0.999 5。平均回收率为103.5%，RSD＜5%。结论 该方法操作简单，准确可靠，适于枸杞多酚提取物中总多酚的测定。

Objective To establish UV-spectrophotometry to determine the content of total polyphenols in polyphenol extract from Lycium barbarum (PELB). Methods The content of total polyphenols in PELB was detected by using UV-spectrophotometry. The sample was diluted with water, chromogenic agent Filin-Ciocalteu was added, the absorbance was detected at 760 nm by UV-spectro- photometry, and with the same method standard curve was measured and drawn. Results The contents of total polyphenols in different batches of PELB were 24.03%, 26.38%, 25.45%, 28.27%, and 29.08%, respectively, the range of linearity was 1―8 μg/mL, and R² = 0.999 5. The average recovery was 103.5% and the RSD was less than 5%. Conclusion The method is proved to be simple, reliable, accurate, repeatable, and could be used to measure the content of total of polyphenols in PELB.