[关键词]
[摘要]
目的 建立高效液相色谱法测定还阳参药材中绿原酸的方法。方法 采用Sino Chrom DDS-BP色谱柱(250 mm×4.6 mm,5 μm),流动相为乙腈-0.4%磷酸水溶液(13∶87),体积流量1.0 mL/min,检测波长327 nm,柱温25 ℃。结果 绿原酸进样量在0.046~0.644 μg与峰面积呈良好的线性关系(r=1),样品平均加样回收率为101.9%,RSD值为1.22%(n=6);检出限和定量限分别为0.01 ng和0.04 ng。结论 此方法简便、准确、重复性好,可用于还阳参药材的质量控制。
[Key word]
[Abstract]
Objective To establish a method of HPLC for the determination of chlorogenic acid in the herbs of Crepis turczaninowii. Methods The samples were separated on a Sino Chrom DDS-BP column (250 mm × 4.6 mm, 5 μm) with the mobile phase of acetonitrile-0.4% phosphoric acid (13:87). The flow rate was 1.0 mL/min; The detection wavelength was set at 327 nm; And column temperature was 25 ℃. Results There was a good linear relationship between the chlorogenic acid and peak area in the range of 0.046—0.644 μg (r = 1). The average recovery rate was 101.9%, and RSD was 1.22% (n = 6). Conclusion The method is simple, accurate, and highly reproductive, and could be used for the quality control of the herbs of C. turczaninowii.
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[基金项目]
山西省中药材、中药饮片地方标准研究项目(2011024A)