目的 建立反相HPLC法测定盐酸米诺环素缓释微丸胶囊中米诺环素的量和有关物质。方法 Diamonsil C₁₈柱（200 mm×4.6 mm，5 μm），流动相为醋酸铵缓冲溶液（pH 6.0）-乙腈-甲醇（80∶15∶5），检测波长为280 nm，体积流量为1.0 mL/min，柱温为25℃，进样量为20 μL。结果 米诺环素在0.05～1 mg/mL线性关系良好（r＝0.999 7），方法精密度RSD为0.6%，平均回收率为99.46%，RSD为0.8%，各杂质峰与主峰分离良好。结论 该方法简便，专属性强，准确可靠，可用于测定盐酸米诺环素缓释微丸胶囊中的米诺环素及有关物质。

Objective To establish an HPLC method for the determination of minocycline hydrochloride and its related substances in minocycline hydrochloride sustained-release pellets capsules. Methods HPLC was performed on a Diamonsil C₁₈ column (200 mm × 4.6 mm, 5 μm) using ammonium acetate (pH 6.0)-acetonitrile-methanol (80:15:5) as the mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was 280 nm and the column temperature was 25 ℃. Results The linear range of minocycline was 0.05—1 mg/mL (r = 0.999 7), precision RSD = 0.6%, and the average recovery was 99.46% (RSD = 0.8%). Conclusion The established method is simple, accurate, and specific, and could be used for the quality control of minocycline hydrochloride sustained-release pellets capsules.