目的 建立高通量检测DPPH自由基清除能力的实验方法，对姜黄提取物抗氧化活性进行初步评价。方法 通过测定抗坏血酸（维生素C）的DPPH自由基清除率曲线，以IC₅₀值作为评价试样清除DPPH自由基能力的指标，并将此应用于测定姜黄醇提物和水提物清除DPPH自由基的能力。结果 测定波长515 nm，抗坏血酸DPPH自由基清除能力在62.5～750.0 μg/mL线性良好，方法可靠。姜黄醇提物的DPPH自由基清除能力（IC₅₀ 7.78 mg/mL）明显强于水提物（IC₅₀ 14.84 mg/mL）。结论 建立的DPPH自由基清除测定方法可靠、简便、灵敏，其高通量的快速检测方法可为抗氧化药物筛选提供参考。

Objective To establish a high-throughput method of DPPH free radical scavenging for evaluating the anti-oxidant activity of the extracts from Curcuma longa. Methods Taking IC₅₀ as the evaluating criterion of DPPH free radical scavenging capability by determining the scavenging rate curves of ascorbic acid, the DPPH free radical scavenging capability of two extracts from C. longa was determined. Results At the wavelength of 515 nm, DPPH free radical scavenging rate of ascorbic acid and concentration could fit a good linearity (0.062 5—0.75 mg/mL), and the results revealed that the method is convenient and reliable. Under this condition, the DPPH free radical scavenging capability of alcohol extracts (IC₅₀ 7.78 mg/mL) was significantly stronger than that of water extracts (IC₅₀ 14.84 mg/mL) from C. longa. Conclusion The convenient and reliable method could be used to evaluate the anti-oxidant activity of other Chinese materia medica, as well as provide an approach and reference for high-throughput activity screening.