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[摘要]
目的 建立可靠稳定的视网膜新生血管动物模型,为探究视网膜新生血管疾病的发生机制和治疗方法奠定模型基础。方法 通过建立氧诱导视网膜新生血管小鼠模型,采用ADP酶染色和视网膜连续切片法,定量评估视网膜新生血管生成情况。结果 模型组小鼠左眼球ADP酶染色结果显示,视乳头周围可见大片无灌注区,并见血管迂曲、扩张、变形,视网膜周边部可见大量新生血管生成;右眼球连续切片发现模型组小鼠有许多明显的血管内皮细胞核突破内界膜,并且内界膜下的细胞增殖,排列紊乱,并见视网膜表面或视网膜前有新生血管芽。结论 成功建立氧诱导视网膜新生血管小鼠模型,并且可作为探究视网膜新生血管疾病发生机制和治疗方法的可靠动物模型。
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[Abstract]
Objective To establish a stable and reliable animal model of retinal neovascularization and to provide a model foundation for further investigation on its mechanism and treatment. Methods Oxygen-induced retina new vessels mouse model were established, and retinal neovascularization was evaluated by ADPase staining and retina sect serial sections. Results ADPase staining showed a pattern of pathological retinal neovascularization such as non-perfused areas, tortuous, dilated blood vessels and deformation in discus opticus of model mice, and retinal neovascularization in retina perimeter. The serial HE staining sections confirmed many conspicuous vascular endothelial cell breakthrough internal limiting membrane, cell proliferation, rank disorder, new vascular bud in retinal surface. Conclusion The oxygen-induced retinal neovascularization mouse model is successfully set up, which could be a reproducible model for the research of mechanism and treatment of retinal neovascularization.
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