目的 建立HPLC-ELSD法测定周络通胶囊中知母皂苷BII的方法。方法 采用ES AquaSep C₁₈（250 mm×4.6 mm，5 μm）色谱柱，以乙腈–水（24∶76）为流动相，体积流量1.0 mL/min；柱温30 ℃；ELSD检测器，漂移管温度为75 ℃，加热级别为66%，载气压力为172.4 kPa。结果 知母皂苷BII在0.62～7.79 μg呈良好的线性关系，平均回收率为98.75%，RSD 1.77%。结论 该方法简便快捷、准确可靠，为周络通胶囊的质量控制提供了依据。

_Objective To establish a method for determining timosaponin BII in Zhouluotong Capsula by HPLC-ELSD. _Methods ES AquaSep C₁₈ column (250 mm × 4.6 mm, 5 μm) was used; The column temperature maintained at 30 ℃ of the ELSD; The mobile phase consisted of acetonitrile-water (24 : 76) at a flow rate of 1.0 mL/min; The temperature of drift tube was 75 ℃; The heating level was 66% and the nebulizernitrogen pressure was 172.4 kPa. _Results Timosaponin BII had a good linearity relationship in the range of 0.62—7.79 μg. The average recovery was 98.75% (RSD = 1.77%). _Conclusion The method is rapid, simple, and accurate, and could be used for quality control of Zhouluotong Capsula.

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