[关键词]
[摘要]
目的:用骨髓微核试验观察苋菜红对小鼠的毒性反应,为进一步致突变研究提供参考。方法:试验动物随机分5组,分别为阴性对照组,苋菜红低、中、高3个剂量组和阳性对照组。每组10只动物,雌雄各5只。连续ig给予不同剂量的苋菜红,观察试验期内小鼠的一般毒性症状、体质量增长情况和骨髓细胞微核率的改变。结果:实验期间动物的精神、外观均无异常。苋菜红高剂量组动物体质量在给药第2~3天下降,中、低剂量组动物体质量未见异常。苋菜红剂量在500~2 000 mg/kg对小鼠骨髓微核率无明显影响。结论:苋菜红对小鼠骨髓细胞无明显致突变作用。
[Key word]
[Abstract]
Objective: Observing toxic reaction and calculating micronucleus rate of mice administrated by different doses of amaranth red to provide a reference for further mutagenesis studies. Methods: The experimental animals were randomly divided into five groups such as negative control group, amaranth red low-, meddle-, and high-dose groups, and positive control group, respectively. Ten animals were in each group, male and female. By continous ig administration, the general toxicity, body weight growth, and bone marrow cell micronucleus in mice were observed during the experimental period. Results: No abnormal reactions in animal mind and appearance were observed. The growth of body weight in high-dose group decreased in days 2-3 after administration, while the animals in low- and middle-dose groups showed normal body weight. Amaranth red within the dose of 500—2 000 mg/kg did not significantly affect the mouse bone marrow micronucleus rate. Conclusion: Amaranth red has no significant mutagenicity on mouse bone marrow cells.
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[基金项目]
中国检验检疫科学研究院基本科研业务费专项资金资助项目(2008JK001),国家质量监督检验检疫总局课题(2009IK310、2008IK133)