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[摘要]
介绍应用荧光定量PCR技术对基因治疗药物组织分布研究的实验方法。从以下几个方面,即定量PCR技术原理、实验设计思路、各种检测方法的比较来说明这一问题。其中,可以使用的检测方法包括紫外定量法和引入内参基因法,后者又可以分为外标法和内标法。充分对比了这些方法的优缺点,并结合本实验室的研究现状,介绍了本监测中心在选用外标法进行研究时所取得的一些成果和实验经验。
[Key word]
[Abstract]
The paper focuses on the test methodology of applying real-time PCR to gene therapy drug biodistribution research. It is illuminated from three aspects containing real-time PCR elements, examination design and various methods comparing. Test results can be detected by ultraviolet and internal control gene method composing of external standard and internal reference techniques. The advantage and deficiency of these methods are fully discussed in this paper, as well as some successful and experimental experiences are reported in the research using external standard method.
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