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[摘要]
目的:利用RP-HPLC方法测定彝族药物固公果中蔷薇酸、委陵菜酸的量。方法:采用Ultimatex B-C18(美国Welch公司,150 nm × 4.6 mm,5 μm)色谱柱,以甲醇-1.5%磷酸水溶液(33︰67)为流动相,体积流量1.0 mL/min,检测波长210 nm。结果:固公果中蔷薇酸与委陵菜酸能得到很好的分离。测定线性范围分别为:蔷薇酸77.4~774.3 μg/mL,r=0.999 9;委陵菜酸54.7~546.9 μg/mL,r=0.999 9。蔷薇酸平均回收率为102.5%(n=9),委陵菜酸平均回收率为103.7%(n=9)。结论:该法便捷、灵敏、准确,重复性好,可以控制该药材的质量。
[Key word]
[Abstract]
Objective: To establish a RP-HPLC method for determination of euscaphic acid and tormertic acid in the root of Rosa odorata var. gigantea. Methods: A Ultimatex B C18 column (150 nm × 4.6 mm, 5 μm) and a mobile phase consisted of methanol-0.15% phosphoric acid (33︰67) were used for analysis. The flow rate was 1.0 mL/min and the UV detector wavelength was 210 nm.Results: Euscaphic acid was well separated from tormertic acid and other compounds in R. odorata var. gigantea. The calibration curves of euscaphic acid and tormertic acid had a good linearity (r=0.999 9) in the range of 0.077 4-0.774 3 mg/mL and 0.054 7-0.546 9 mg/mL, respectively. The average recovery of euscaphic acid and tormertic acid were 102.5% (n=9) and 103.7% (n=9). Conclusion: This method is simple, accurate, and reproducible, which is suitable for the quality control of euscaphic acid and tormertic acid in the roots of .R. odorata var. gigantea.
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[基金项目]
天津市自然科学基金项目(10JCYBJC14600)资助。