目的：建立测定血浆中委陵菜黄酮的分析方法，初步对该化合物在大鼠体内的经时过程进行考察。方法：建立委陵菜黄酮的HPLC测定方法，考察大鼠iv委陵菜黄酮（10、20、40 mg/kg）后血药浓度-时间曲线。结果：大鼠尾iv 3 个剂量委陵菜黄酮后，血浆药时曲线符合三室模型，药时曲线下面积AUC_0-180分别为346.05、455.74和1 655.14 μg/（mL·min），分布相半衰期t_1/2α分别为103.90、15.59和9.49 min，消除相半衰期t_1/2β分别为30 580.98、2 045.70和894.84 min。结论：iv给药后，委陵菜黄酮血浆药时曲线符合三室模型，在大鼠体内迅速分布，但消除速率较慢。

Objective: To establish the method of measuring concentration of flavones from Potentilla chinensis in plasma of rats and to explore its pharmacokinetic features. Methods: An HPLC analysis method for the determination of flavones in P. chinensis was established and used to analyze the plasma concentration-time curve on iv injection of flavones in P. chinensis to rats. Results: A RP-HPLC analysis method with C-18 column for the determination of flavones in P. chinensis was established. After the injection of 10, 20, and 40 mg/kg of flavones in P. chinensis, the plasma concentration-time curve was in line with the three-compartment model, AUC0-180 were 346.05, 455.74, and 1 655.14 μg/(mL·min), respectively. Distribution phase half-life t_1/2α was 103.90, 15.59, and 9.49 min, and elimination phase half-life t_1/2β were 30 580.98, 2 045.70, and 894.84 min, respectively. Conclusion: After iv administration, the plasma concentration-time curve of flavones in P. chinensis is in line with the three-compartment model, the rapid distribution, while the slower rate of elimination in rats.

教育部博士点基金项目(200800620007)