[关键词]
[摘要]
目的:分析胃肠安丸方中的泻下成分与止泻成分,并对具有泻下作用的大黄蒽醌进行定量测定。方法:利用LC-MS对胃肠安丸复方中的主要成分进行定性分析。色谱柱:Kromasil C18色谱柱(250 mm×4.6 mm,5 μm);流动相:甲醇-0.5%醋酸溶液梯度洗脱;体积流量1.0 mL/min;检测波长254 nm。结果:芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚总质量分数在1.03 mg/g以上,平均回收率为100.38%,所得结果的RSD值均小于2%。结论:LC-MS分析确定了胃肠安丸中的12个成分,其中既有止泻作用成分又有泻下作用成分,对其中具有明显泻下作用的蒽醌类化合物进行定量检测,方法简便、灵敏、准确,为探讨胃肠安丸的作用物质基础提供数据支持。
[Key word]
[Abstract]
Objective: To analyze the main components and establish an HPLC method for determination of free anthraquinones content in Weichang’an Pill. Methods: The separation was performed on ODS column (250 mm × 4.6 mm, 5 μm) of Kromasil. The mobile phase was a mixture of methanol-0.5% acetic acid solution, gradient elution, the flow rate was 1.0 mL/min and the wavelength was at 254 nm. Results: Twelve components of Weichang’an Pill were identified, including antidiarrhea and purgative constituents, simultaneously the total content of aloe emodin, rhein, emodin, chrysophanol, and physcion was determined at 1.03 mg/g. The average recovery rate was 100.38%, RSD of the results are all less than 2%. Conclusion: The method is simple, accurate, sensitive, and can be used to provide the data for the quality control of Weichang’an Pill.
[中图分类号]
[基金项目]