[关键词]
[摘要]
目的:冰片和薄荷脑是清咽滴丸中主要挥发性有效成分。本研究拟建立大鼠ig清咽滴丸后血浆中龙脑和薄荷脑同时测定的气相色谱分析方法,阐明清咽滴丸中龙脑、薄荷脑大鼠体内药代动力学特征。方法:血浆样品采用内标法并经液-液萃取处理,同时采用氢火焰离子化检测器(FID)检测龙脑和薄荷脑大鼠体内的血药浓度。结果:本法可同时测定龙脑、薄荷脑的大鼠血浆浓度,并采用DAS(Version 1.0)软件拟合药代动力学参数,结果表明龙脑和薄荷脑均符合开放性一室模型。龙脑在2.5~50.0 ng/μL(r=0.996 3),薄荷脑在8.7~62.2 ng/μL(r=0.999 4)均具有良好的线性关系,最低定量限分别为2.4、5.0 ng/μL。结论:建立了灵敏、简便、准确的气相色谱法,该方法适用于清咽滴丸中冰片和薄荷脑体内药代动力学研究,为清咽滴丸制剂的临床合理用药提供了理论依据。
[Key word]
[Abstract]
Objective: Borneol and menthol are both main active substances of Qingyan Drop Pills. To establish a method for simultaneous determination of borneol and menthol in plasma of rats after ig administration of Qingyang Drop Pills by gas chromatography. Methods: The blood sample was carried out by liquid-liquid extraction (LLE) with an internal standard solution in naphthalene. Borneol and menthol were determined by means of flame ionization detection (FID). Results: The pharmacokinetic parameters were estimated by a compartmental method using the DAS software program (Version 1.0). The standard curves were linear over a wide concentration rang of 2.5 ? 50.0 ng/μL (r=0.996 3), 8.7 ? 62.2 ng/μL (r=0.999 4) for both borneol and menthol in plasma, respectively. The limit of quantification (LOQ) of borneol and menthol in plasma was 2.4 and 5.0 ng/μL, respectively. Conclusion: This validated assay method has been successfully applied to a pharmacokinetic study of borneol and menthol in plasma of rats after ig administration of Qingyan Drop Pills. The pharmacokinetic parameters provide some information for clinical administration of Qingyan Drop Pills.
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[基金项目]
国家自然科学基金(项目编号:30772778)、国家科技支撑计划(项目编号:2007BAI47B04)。