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[摘要]
目的:观察丹参酮ⅡA对阿霉素诱导2种肿瘤细胞(K562和Bel-7402细胞)死亡作用的影响。方法:MTT法检测细胞存活率,Hoechst 33342染色从形态学上反映肿瘤细胞凋亡情况。结果:丹参酮ⅡA本身对2种肿瘤细胞均有抑制增殖、促凋亡的作用,不会干扰阿霉素诱导肿瘤细胞死亡的作用,甚至对其对K562细胞的促凋亡作用有协同效果。结论:丹参酮ⅡA对阿霉素抑制肿瘤细胞生长有协同作用,但对肿瘤细胞有一定的选择性。
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[Abstract]
Objective: To investigate the effects of TanshinoneⅡA on Adriamycin-induced suppression of growth in two kinds of tumor cell (K562 and Bel-7402). Methods: MTT colorimetric assay was used to detect the survival rate of tumor cells. Hoechst 33342 staining was used to reflect tumor cells apoptosis morphologically after different treatments. Results: Tanshinone IIA alone induced cell death in K562 cells and human hepatocarcinoma Bel-7402 cells. It did not interfere with the antiproliferative effects of Adriamycin in these two cells. When used with Adriamycin, Tanshinone IIA even enhanced cell death induced by Adriamycin in K562 cells. Conclusion: Tanshinone IIA showes synergistic effect with Adriamycin in suppression of tumor cells growth, however, with cell type specificity.
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