目的：建立参茸健脾胶囊中10-羟基-2-癸烯酸的HPLC测定方法。方法：采用Agilent 1100 DAD高效液相色谱仪，Agilent Extend C₁₈色谱柱（250 mm×4.6 mm，5 μm），乙腈-0.1％磷酸（23∶77）为流动相，检测波长为207 nm，流速1 mL/min。结果：10-羟基-2-癸烯酸在0.035 4～0.424 3 μg范围内线性关系良好（r＝0.999 5），平均回收率为100.6％，RSD＝1.9％ 。结论：该法简便、快速、准确，适用于参茸健脾胶囊中10-羟基-2-癸烯酸的定量测定，有利于控制参茸健脾胶囊的质量。

Objective: To establish an HPLC method for simulataneous determination of 10-hydroxy-2-decenoic acid in Shenrong Jianpi Capsula. Methods: The HPLC was conducted on discovery Agilent Extend C₁₈ column (250 mm × 4.6 mm, 5 μm). with acetonitrile-0.1% phosphoric acid solution (23:77) as the mobile phase. The detection wavelength was 207 nm, and flow rate was 1 mL/min. Results: There were a good linear relationship of 10-hydroxy-2-decenoic acid within 0.035 4—0.424 3 μg (r = 0.999 5). The average recovery rate was 100.6%, RSD was 1.9%. Conclusion: The method is simple, rapid and accurate for the determination of 10-hydroxy-2-decenoic acid in Shenrong Jianpi Capsula, and it is expected? for the quality control of Shenrong Jianpi Capsula.