[关键词]
[摘要]
目的:研究建立血小板沉积模型的方法。方法:采用99锝体外血小板标记与光化学血管内膜损伤法建立模型,通过血小板沉积数测定研究模型的应用性。结果:实验结果表明,99锝体外血小板标记率受标记时间影响,最佳时间为30 min;洗涤2次可将未标记同位素基本分离掉;血小板沉积数表示法较直接CPM计数法优越;阳性对照欣维宁可减少血小板在内膜损伤动脉血管壁上的沉积。结论:通过99锝体外血小板标记与光化学血管内膜损伤建立的血小板沉积方法,科学、可靠,有一定实用性。
[Key word]
[Abstract]
Objective: To study the method of the model establishment for platelet deposition. Methods: Using 99mTc labeled platelet and damage of vascular intima with photo- chemistry, the application of model for platelet deposition was studied by the measurement of platelet deposition. Results: The labeling ratio of platelet with 99mTc was affected by the labeling time, the optimal time was 30 min, the unlabeled platelet could be separated from labeled platelet through two times of scrubbing by buffer, the method of the counts of platelet deposition has superiority than that of CPM, the deposition of platelet in damaged vessels with photochemistry could be reduced by Tirofiban in the positive control group. Conclusions: The method of 99mTc labeled platelet in vitro and damage of vascular intima with photochemistry is reliable, and can be used for evaluation of antiplatelet agents.
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[基金项目]
系列化、国际化的国家生物医药国际创新园新药研发综合性大平台建设资助项目(009ZX09301-008)。