目的：研究藻红蛋白对人乳腺癌 MCF-7 细胞的诱导衰老作用。方法：采用 MTT 法检测藻红蛋白对 MCF-7 细胞的生长抑制作用，采用倒置显微镜观察形态学变化，酸性 β-半乳糖苷酶染色法和衰老相关异染色质聚集检测藻红蛋白诱导人乳腺癌 MCF-7 细胞衰老作用。结果：MTT 法结果显示，藻红蛋白对体外培养的人乳腺癌细胞 MCF-7 具有明显的生长抑制作用，IC₅₀ 为 134.87 μg/mL。结果发现藻红蛋白作用 72 h 后，细胞呈现变大变扁平，细胞内形成空泡、颗粒增多等明显衰老形态。在倒置显微镜下观察，酸性 β-半乳糖苷酶染色呈阳性；在荧光显微镜下观察，DAPI 染色出现衰老相关异染色质聚集现象。结论：藻红蛋白可通过诱导 MCF-7 细胞衰老达到抗肿瘤作用。

Objective: To investigate the effects of phycoerythrin（PE）on the induction of cell senescence in human breast cancer MCF-7 cells. Methods: The inhibitory rate of MCF-7 cells growth was measured by MTT assay and morphological changes of MCF-7 cells was observed through the inverted microscope. Acidic β-galactosidase dying (SA-β-Gal dying) and senescence-associated heterochromatin aggregation were used to detect the cell senescence caused by PE in MCF-7 cells. Results: The results of MTT assay indicates that PE could inhibit the growth of human breast cancer MCF-7 cells significantly and the IC₅₀ was 134.87 μg/mL. After 72 h, MCF-7 cells which were treated by PE had become bigger and flatter meanwhile the appearance of vacuolization and particles which are the specific patterns of the cell senescence were also observed. The staining of acidic β-galactosidase was positive, which observed by inverted microscope, and, the emergence of senescence-associated heterochromatin aggregation (SAHF) in staining of DAPI was found under the fluorescence microscope.Conclusion: The results demonstrate that PE has the antitumor effect by inducing the cell senescence of MCF-7 cells.

国家自然科学基金项目（300672595）；教育部科学技术研究重点项目（207030）；教育部博士点基金项目（20070240081）；黑龙江省自然科学基金重大项目（ZJY0504）