目的 通过体外人脐静脉内皮细胞和海绵植入小鼠模型对丹参酮Ⅱ_A磺酸钠的促进血管新生作用进行研究。方法 体外采用人脐静脉内皮细胞HUVEC-12诱导模型，利用细胞迁移及小管生成实验评价丹参酮Ⅱ_A磺酸钠促血管新生的作用影响；体内实验采用海绵植入小鼠模型，通过QPCR测定不同剂量丹参酮Ⅱ_A磺酸钠对VEGF-A mRNA表达的影响，采用免疫荧光染色法观察不同剂量丹参酮Ⅱ_A磺酸钠对微血管生成及VEGF蛋白释放的影响。结果 丹参酮Ⅱ_A磺酸钠40、80 μg/mL剂量组的迁移率显著高于对照组（P<0.05、0.01）；丹参酮Ⅱ_A磺酸钠与形成的结节数目呈现一定剂量相关性，且丹参酮Ⅱ_A磺酸钠80 μg/mL剂量组的小管生成节结数显著高于对照组（P<0.05）。丹参酮Ⅱ_A磺酸钠12、24 mg剂量组VEGF-A mRNA相对表达值显著高于对照组（P<0.01），而丹参酮Ⅱ_A磺酸钠12 mg剂量组作用最强，优于阳性药丹参多酚酸盐。丹参酮Ⅱ_A磺酸钠12、16、24 mg剂量组的VEGF蛋白表达量明显优于对照组（P<0.05、0.01），丹参酮Ⅱ_A磺酸钠12 mg剂量组，VEGF蛋白表达量最高，荧光面积最大，优于阳性药丹参多酚酸盐。结论 丹参酮Ⅱ_A磺酸钠具有促血管新生作用，其作用机制可能通过上调VEGF-A mRNA的表达来影响。

Objective To study the effect of angiogenesis effect of sodium tanshinone Ⅱ_A sulfonate on human umbilical vein endothelial cell in vitro and sponge implantation in rats. Methods HUVEC-12 induction model was selected in vitro, and the effect of sodium tanshinone Ⅱ_A sulfonate on angiogenesis was evaluated by cell migration and tubule formation. Sponge implantation assay was selected in vivo, and the effect of sodium tanshinone Ⅱ_A sulfonate in different doses on the expression of VEGF-A mRNA was detected by QPCR. The effects of sodium tanshinone Ⅱ_A sulfonate in different doses on microvascular production and VEGF protein release were observed by immunofluorescence staining. Results The migration rate in sodium tanshinone Ⅱ_A sulfonate 40 and 80 μg/mL group were significantly higher that in the control group (P < 0.05, 0.01). Sodium tanshinone Ⅱ_A sulfonate had the dosage association with the number of tubule formation, and the number of tubule formation in sodium tanshinone Ⅱ_A sulfonate 80 μg/mL group was significantly higher that in the control group (P < 0.05). VEGF-A mRNA expression in sodium tanshinone Ⅱ_A sulfonate 12 and 24 mg group were significantly higher that in the control group (P < 0.01). VEGF-A mRNA expression in sodium tanshinone Ⅱ_A sulfonate 12 mg group was the highest, and higher than that in salvianolate group. VEGF protein expression in sodium tanshinone Ⅱ_A sulfonate 12, 16, and 24 mg group was significantly higher that in the control group (P < 0.05, 0.01). VEGF protein expression in sodium tanshinone Ⅱ_A sulfonate 12 mg group was the highest, and fluorescence area was the biggest, and better than that in salvianolate group. Conclusion Sodium tanshinone Ⅱ_A sulfonate has the effect of promoting angiogenesis, which is related to promoting the expression of VEGF-A mRNA mainly.