天然产物是药物发现的重要资源，但其靶点不明的问题在一定程度上限制了后续的精准优化和新药研发。天然产物靶点的发现有助于阐明其作用机制、加速新药研发进程。天然产物靶点发现技术取得了革命性进步。总结了天然产物靶点发现的主要技术，包括基于亲和力的标记技术、基于蛋白稳定性的无标记技术，以及基于降解的蛋白质组学分析技术，阐述了技术原理、优势、局限性以及代表性应用案例，旨在为天然产物靶点鉴定、机制解析提供参考。

Natural products are important resources for drug discovery, but the problem of unclear targets to some extent limits subsequent precise optimization and new drug development. The discovery of natural product targets helps to elucidate their mechanisms of action and accelerate the development of new drugs. The discovery technology of natural product targets has made revolutionary progress. This article summarizes the main technologies for discovering natural product targets, including affinity based labeling techniques, protein stability based label free techniques, and degradation based proteomics analysis techniques. It elaborates on the technical principles, advantages, limitations, and representative application cases, aiming to provide reference for the target identification and mechanism analysis of natural products.

R965.2

北京市自然科学基金项目(7252200)