目的 建立高效液相色谱法同时测定不同产地北沙参中欧前胡素、异欧前胡素和法卡林二醇。方法 采用Zorbax Eclipse Plus C₁₈色谱柱（250 mm×4.6 mm，5 μm）；流动相为乙腈–0.05%磷酸水，梯度洗脱；体积流量1.0 mL/min；柱温：30 ℃；检测波长：248 nm；进样量：20 μL。对河北、山东、内蒙古3大主产地20批北沙参样品进行测定。结果 欧前胡素、异欧前胡素、法卡林二醇在0.12～12.36、0.12～12.02、1.24～124.48μg/mL线性关系良好。内蒙古产北沙参中欧前胡素、异欧前胡素总量略高于山东、河北，法卡林二醇的量以内蒙古和山东相近，均高于河北。结论 方法简便、准确、可靠，可用于北沙参的定量分析。

Objective To establish an HPLC method for simultaneous determination of imperatorin, isoimperatorin, and falcarindiol in Glehniae Radix from different habitats. Methods Zorbax Eclipse Plus C₁₈ column (250 mm × 4.6 mm, 5 μm) was used, with acetonitrile - 0.05% phosphoric acid water as the mobile phase with gradient elution, the flow rate was 1.0 mL/min, the detection wavelength was set at 248 nm, and the injection volume was 20 μL. And the contents of 20 batches of Glehniae Radix samples from three main producing areas (Hebei, Shandong, and Inner Mongolia) were determined. Results Imperatorin, isoimperatorin and falcarindiol showed a good linear relationship in the range of 0.12—12.36, 0.12—12.02, and 1.24—124.48 μg/mL. The total content of imperatorin and isoimperatorin in Glehniae Radix from Inner Mongolia was slightly higher than that from Shandong and Hebei, and the content of falcarindiol in Inner Mongolia was similar to that in Shandong, both higher than that in Hebei. Conclusion The method is simple, accurate and reliable, which can be used for the determination of Glehniae Radix.

R284.1

山东省重点研发计划-乡村振兴科技创新提振行动计划(2023TZXD081)