目的 研究不同炮制时间茅根炭超高效液相色谱（UPLC）指纹图谱与饮片粉末色度值的关系。方法 以特征峰相对峰面积、色度值为指标进行化学计量学分析，进行粉末色度值与各特征峰相对峰面积相关性分析。结果 UPLC指纹图谱的11个特征峰相对峰面积与色度值呈现不同程度的相关性：其中新绿原酸、化合物5、咖啡酸、绿原酸、化合物8、化合物9和隐绿原酸随着炭化程度加深而含量递减，化合物1、5-羟甲基糠醛、化合物3、4-香豆酸随着炭化程度加深而含量先增后减。聚类分析（HAC）、主成分分析（PCA）、正交偏最小二乘法判别分析（OPLS-DA）均将不同炮制程度的茅根炭分为3类，OPLS-DA结果显示5-羟甲基糠醛、隐绿原酸、4-香豆酸、绿原酸和化合物5是茅根炭炮制过程中的重要质量评价指标。结论 茅根炭粉末色度值与化学成分变化的关联度较高，可为茅根炭质量控制研究提供思路。

Objective To investigate the relationship between UPLC fingerprints and chromaticity values of carbonized Imperatae Rhizoma with different processing times. Methods Chemometrics analysis was conducted using the relative peak area of characteristic peaks and chromaticity values as indicators. The correlation between the chromaticity values of the powder and the relative peak areas of each characteristic peak was analyzed. Results The relative peak areas of 11 characteristic peaks in the UPLC fingerprint showed different degrees of correlation with chromaticity values: among them, the contents of neochlorogenic acid, compound 5, caffeic acid, chlorogenic acid, compound 8, compound 9, and cryptochlorogenic acid decreased with the deepening of carbonization degree. While the contents of compound 1, 5-HMF, compound 3, and 4-coumaric acid first increased and then decreased as the carbonization degree deepened. Carbonized Imperatae Rhizoma with different processing degrees were all classified into 3 categories according to HCA, PCA, and OPLS-DA. The OPLS-DA results indicated that 5-HMF, cryptochlorogenic acid, 4-coumaric acid, chlorogenic acid, and compound 5 were important quality indicators during the processing of carbonized Imperatae Rhizoma. Conclusion There is a high correlation between the chromaticity value of carbonized Imperatae Rhizoma powder and its chemical composition changes, which can provide ideas for the quality for carbonized Imperatae Rhizoma.

R286.02

佛山市南海区重点领域科技攻关专项（南科[2023]20 号-18）