[关键词]
[摘要]
目的 从铁死亡角度探讨藁本内酯减轻脑微血管内皮细胞（BMECs）缺糖缺氧/复氧（OGD/R）损伤。方法 体外构建OGD/R模型，酶联免疫吸附（ELISA）试剂盒检测还原型谷胱甘肽（GSH）、铁死亡相关蛋白谷胱甘肽过氧化物酶4（GPX-4）、4-羟壬二酸酯（4-HNE）和细胞内铁含量变化。采用流式细胞术检测细胞内脂质活性氧（ROS）水平变化；Western blotting法检测GPX-4、溶质载体家族7成员11（SLC7A11）的蛋白表达水平变化。结果 与模型组相比，藁本内酯各浓度组和铁抑素-1（Fer-1）组可显著升高GPX-4、GSH含量及SLC7A11、GPX-4 蛋白表达水平；4-HNE、ROS、铁含量显著降低（P＜0.05、0.01）。结论 藁本内酯可通过抑制脑微血管内皮细胞铁死亡改善缺糖缺氧/复氧导致的细胞损伤。
[Key word]
[Abstract]
Objective To explore ligusticide in alleviating OGD/R injury in BMECs from the perspective of ferroptosis. Methods OGD/R model was constructed in vitro, the contents of reduced GSH, GPX-4, 4-HNE, and iron were detected by ELISA. The changes of lipid ROS level was detected by flow cytometry. The protein expression levels of GPX-4 and SLC7A11 were detected by Western blotting. Results Compared with model group, ligustilide groups and the Fer-1 group could significantly increase the contents of GPX-4 and GSH, and protein expression levels of SLC7A11 and GPX-4, the contents of 4-HNE, ROS and iron were significantly reduced (P < 0.05, 0.01). Conclusion Ligustilide can improve the damage of cerebral microvascular endothelial cells caused by hypoxia/reoxygenation by inhibiting ferroptosis.
[中图分类号]
R286.1
[基金项目]
安徽省第十三批 115 产业创新团队“针药结合防治脑病产业创新团队”项目（皖人才办[2020]4 号）； 安徽省高等学校科学研究项目（2024AH051015）；安徽省高校优秀科研创新团队计划（2022AH010034）；安徽中医药大学校级科学研究项目（2020sfyzc02）
