目的 采用多指标成分定量联合PCA、OPLS-DA和CRITIC-TOPSIS法评价臭灵丹草质量差异。方法 选取臭灵丹草中金丝桃苷、木犀草素、芹菜素、洋艾素、金腰乙素、3,4-O-二咖啡酰奎宁酸、4,5-O-二咖啡酰奎宁酸、3,5-O-二咖啡酰奎宁酸、臭灵丹二醇、冬青叶豚草酸、臭灵丹酸、水溶性浸出物、总灰分和酸不溶性灰分为质量评价指标，以PCA和OPLS-DA法对15批臭灵丹草进行分组，筛选质量差异标志物；以CRITIC法计算各指标权重，采用TOPSIS法构建臭灵丹草综合质量评价模型。结果 不同产地臭灵丹草中14个指标含量差异较大；OPLS-DA分析可将15批臭灵丹草按照不同产地分为3类，筛选出臭灵丹酸、3,5-O-二咖啡酰奎宁酸、洋艾素、冬青叶豚草酸和金腰乙素为质量差异标志物；CRITIC-TOPSIS法结果显示15批样品综合质量评价指标的相对接近度最大值为0.632 1，最小值为0.392 7；云南产臭灵丹草样品排序靠前。结论 PCA、OPLS-DA和CRITIC-TOPSIS法可用于臭灵丹草的质量差异评价，并且不同产地臭灵丹草药材质量存在差异。

Objective To evaluate the quality difference of Laggerae Herba based on multi-index component quantitation combined with PCA, OPLS-DA, and CRITIC-TOPSIS method. Method The contents of 14 quality evaluation indexes, including hyperoside, luteolin, apigenin, artemitin, chrysosplenetin B, 3,4-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, pterodondiol, ilicic acid, pterodontic acid, water soluble extract, total ash, and acid insoluble ash were selected. PCA and OPLS-DA methods were used to group 15 batches of Laggerae Herba, and the quality difference markers were screened. The CRITIC method was used to calculate the weight of each index, and the TOPSIS method was used to construct the comprehensive quality evaluation model of Laggerae Herba. Results The contents of 14 indexes in Laggerae Herba from different producing areas were quite different. OPLS-DA analysis could divide 15 batches of Laggerae Herba into three categories according to different producing areas. Pterodontic acid, 3,5-O-dicaffeoylquinic acid, artemitin, ilicic acid, and chrysosplenetin B were selected as quality difference markers. The results of CRITIC-TOPSIS method showed that the maximum relative proximity of the comprehensive quality evaluation indexes of 15 batches of samples was 0.632 1, and the minimum value was 0.392 7. The rank of Laggerae Herba samples from genuine producing areas Yunnan was front. Conclusion PCA, OPLS-DA, and CRITIC-TOPSIS can be used to evaluate the quality difference of Laggerae Herba, and there are differences in the quality of Laggerae Herba from different producing areas.

R284

河南省医学科技攻关计划联合共建项目（LHGJ20230989）