目的 探讨红景天苷对顺铂诱导小鼠急性肾损伤的保护作用及机制。方法 将SPF级昆明小鼠随机分为对照组、模型组、红景天苷组、顺铂＋红景天苷组，每组7只。红景天苷组和顺铂＋红景天苷组小鼠ig 150 mg/kg红景天苷2周，其余组ig等量生理盐水，自第11天起，模型组和顺铂＋红景天苷组ip 10 mg/kg顺铂诱导小鼠急性肾损伤模型，其余组ip等量生理盐水。各组检测血清肌酐（Scr）和尿素氮（BUN）水平，肾脏病理组织学变化，小鼠Toll-样受体4（TLR4）、髓样分化因子88（Myd88）、核因子κB（NF-κB）mRNA及蛋白表达。结果 与顺铂组比较，顺铂＋红景天苷组小鼠Scr与BUN水平显著降低（P＜0.05），小鼠肾脏损伤明显改善，TLR4、Myd88和NF-κB mRNA及蛋白表达显著降低（P＜0.05）。结论 红景天苷对顺铂导致的急性肾损伤具有保护作用，其机制可能与抑制TLR4/Myd88/NF-κB通路的激活有关。

Objective To explore the protective effect and mechanism of salidroside against cisplatin-induced acute kidney injury in mice. Methods SPF Kunming mice were randomly divided into control group, model group, salidroside group, cisplatin + salidroside group, with 7 mice in each group. Cisplatin and cisplatin + salidroside groups were ig 150 mg/kg salidroside for 2 weeks, and the remaining groups were treated with equivalent amounts of saline. Since day 11, model and cisplatin + salidroside groups groups received 10 mg/kg cisplatin-inducedacute kidney injury model, and the remaining groups were given equal amounts of saline. Scr and levels, pathological histological changes in kidney, TLR4, Myd88 and NF-κB mRNA and protein expression in mice were measured. Results Compared with cisplatin group, Scr and BUN levels were significantly decreased (P < 0.05), kidney injury were improved, and the mRNA and protein expression of TLR4, Myd 88, NF- κB were significantly decreased (P < 0.05). Conclusion Salidroside has a protective effect on acute kidney injury caused by cisplatin, and the mechanism may be related to the inhibition of the activation of the TLR4/Myd88/NF-κB pathway.

R285.5

湖南省大学生创新创业训练计划项目（S202310531044）