目的 探究2-O-葡萄糖基白及苷对糖尿病小鼠肾脏的保护作用及其对Toll样受体4/核转录因子-κB/NOD样受体蛋白3（TLR4/NF-κB/NLRP3）通路的影响。方法 将C57BL/6小鼠随机分为对照组、模型组、厄贝沙坦组、2-O-葡萄糖基白及苷组，每组10只。检测小鼠体质量、肾质量、肾指数；通过HE染色、PAS染色、Masson染色观察肾脏病理形态；透射电镜观察肾脏超微结构；检测小鼠血清糖代谢指标[胰岛素（INS）、空腹血糖、糖化血红蛋白（GHb）]、肾功能指标[血尿素氮（BUN）、血肌酐（SCr）和尿白蛋白（ALB）]、炎性因子［白细胞介素（IL）-1β、IL-6、肿瘤坏死因子-α（TNF-α）］水平以及蛋白免疫印迹法检测肾组织中TLR4、NF-κB p65、p-NF-κB p65和NLRP3蛋白的表达水平。结果 2-O-葡萄糖基白及苷干预后，肾组织病理形态改善，间质纤维化程度减轻，基底膜厚度变薄，空腹血糖降低（P＜0.05）；BUN、SCr、ALB水平降低（P＜0.05）；IL-1β、IL-6、TNF-α水平降低（P＜0.05）；TLR4、NLRP3和p-NF-κB p65/NF-κB p65蛋白表达水平显著降低（P＜0.05）。结论 2-O-葡萄糖基白及苷能够减轻糖尿病小鼠肾脏损伤状态，调节血糖，改善肾功能，减轻炎症反应，其机制可能与抑制TLR4/NF-κB/NLRP3通路有关。

Objective To investigate the renal protective effect of 2-O-glucosyl bletilla striata glycoside on diabetic mice, and its effect on TLR4/NF-κB/NLRP3 pathway. Methods C57BL/6 mice were randomly divided into control group, model group, irbesartan group, and 2-O-glucosyl bletilla striata glycoside group, with 10 mice in each group. Body weight, kidney weight, and kidney index were measured sequentially. Renal pathological morphology was assessed using HE staining, PAS staining, and Masson staining, while renal ultrastructure was examined via transmission electron microscopy. Serum glucose metabolism indices (INS, fasting blood glucose, GHb), renal function indices (BUN, SCr, ALB), and levels of inflammatory factors (IL-1β, IL-6, TNF-α) were measured. The expression levels of TLR4, NF-κB p65, p-NF-κB p65, and NLRP3 proteins in renal tissue were assessed using Western blotting. Results After 2-O-glucosyl bletilla striata glycoside intervention, the pathological morphology of renal tissue improved, the degree of interstitial fibrosis was alleviated, and the thickness of basement membrane became thinner, fasting blood glucose decreased (P < 0.05). BUN, SCr, and ALB levels were decreased (P < 0.05), IL-1β, IL-6, and TNF-α levels were decreased (P < 0.05). TLR4, NLRP3 and p-NF-κB p65/NF-κB p65 protein expressions were decreased (P < 0.05). Conclusion 2-O-glucosyl bletilla striata glycoside can alleviate renal damage in diabetic mice, regulate blood sugar, improve renal function, and reduce inflammatory response, and its mechanism may be related to the inhibition of TLR4/NF-κB/NLRP3 pathway.

R285.5;R286.7

云南省科技厅科技计划项目（202101AZ070001-046）