目的 建立HPLC-水凝粒子激光计数检测器（NQAD）法测定芪冬颐心颗粒中黄芪甲苷、麦冬皂苷D、丹参酮Ⅱ_A、人参皂苷Rb₁、茯苓酸、梓醇、桂皮醛、淫羊藿苷、绿原酸和柚皮苷。方法 使用InfinityLab Prooshell 120 SB-C₁₈色谱柱（150 mm×4.6 mm，4 μm）；流动相：0.1%甲酸-乙腈，梯度洗脱；柱温：40 ℃；进样量：10 µL；体积流量：0.9 mL/min；NQAD参数为蒸发温度：90 ℃；喷雾温度：30 ℃；倍率：1；出口体积流量：1.4 mL/min；平滑度：5 s；线性补正：开。结果 黄芪甲苷、麦冬皂苷D、丹参酮Ⅱ_A、人参皂苷Rb₁、茯苓酸、梓醇、桂皮醛、淫羊藿苷、绿原酸和柚皮苷的平均回收率分别为98.88%、97.81%、97.23%、96.79%、98.11%、97.21%、98.08%、97.98%、97.38%、97.72%，RSD值分别为0.86%、1.14%、1.27%、1.32%、1.05%、1.42%、0.99%、1.11%、1.17%、1.47%。结论 方法高效、快速、准确，可以应用于芪冬颐心颗粒的质量控制。

Objective To develop an HPLC-NQAD method for determination of astragaloside, ophiopogonin D, tanshinone Ⅱ_A, ginsenoside Rb₁, pachymic acid, catalpol, cinnamaldehyde, icariin, chlorogenic acid, and naringin in Qidong Yixin Granules. Methods The chromatographic separation was performed on InfinityLab Prooshell 120 SB-C₁₈ column (150 mm × 4.6 mm, 4 μm) with a gradient elution of 0.1% formic acid-acetonitrile. Column temperature was 40 ℃, injection volume was 10 µL, and volumetric flow rate was 0.9 mL/min. The NQAD parameter was as following: evaporation temperature: 90 ℃, spray temperature: 30 ℃, magnification: 1, export volume flow rate: 1.4 mL/min, smoothness: 5 s, and linear correction: on. Results The average recoveries of astragaloside, ophiopogonin D, tanshinone Ⅱ_A, ginsenoside Rb₁, pachymic acid, catalpol, cinnamaldehyde, icariin, chlorogenic acid and naringin were 98.88%, 97.81%, 97.23%, 96.79%, 98.11%, 97.21%, 98.08%, 97.98%, 97.38%, and 97.72%, respectively, with RSD values of 0.86%, 1.14%, 1.27%, 1.32%, 1.05%, 1.42%, 0.99%, 1.11%, 1.17%, and 1.47%, respectively. Conclusion The method is efficient, fast, and accurate, and can be applied to the quality control of Qidong Yixin Granules.

R286.02