目的 采用超高效液相色谱-一测多评（UPLC-QAMS）法测定生姜总酚软胶囊中6-姜辣素、8-姜酚、6-姜烯酚、10-姜酚。方法 采用Acquity UPLC HSS T3 C₁₈色谱柱（100 mm×2.1 mm，1.8 μm）；流动相为乙腈–水，梯度洗脱；检测波长：220 nm；体积流量为0.3 mL/min；柱温为30 ℃；进样量1 μL。以6-姜辣素为内参物，计算8-姜酚、6-姜烯酚、10-姜酚的相对校正因子。结果 6-姜辣素、8-姜酚、6-姜烯酚、10-姜酚的平均回收率分别为101.10%、100.98%、100.39%、101.32%，RSD值分别为2.12%、2.00%、2.40%、2.03%。采用外标法与QAMS法计算生姜总酚软胶囊中6-姜辣素、8-姜酚、6-姜烯酚、10-姜酚的检测结果无显著差异。结论 方法准确性高、重复性好，可为生姜总酚软胶囊的质量控制提供参考。

Objective To establish an analysis method of 6-gingerol, 8-gingerol, 6-shogaol and 10-gingerol in Ginger Total Phenol Soft Capsules by UPLC-QAMS method. Methods The separation was performed on Acquity UPLC HSS T3 C₁₈ chromatographic column (100 mm×2.1 mm, 1.8 μm) with gradient elution using acetonitrile - water as the mobile phase. The detection wavelength was set at 220 nm, the flow rate was 0.3 mL/min, the column temperature was 30 ℃, and the injection volume was 1 μL. Using 6-gingerol as the internal reference substance, the relative correction factors of 8-gingerol, 6-shogaol and 10-gingerol were calculated. Results The average recoveries of 6-gingerol, 8-gingerol, 6-shogaol and 10-gingerol were 101.10%, 100.98%, 100.39%, and 101.32%, respectively, with RSD values of 2.12%, 2.00%, 2.40%, and 2.03%. There were no significant differences in the detection results of 6-gingerol, 8-gingerol, 6-shogaol and 10-gingerol in Ginger Total Phenol Soft Capsules when calculated using the external standard method and the QAMS method. Conclusion The method exhibits high accuracy and good repeatability, providing a reference for the quality control of Ginger Total Phenol Soft Capsules.

R286.02

国家药监局中药材质量监测与评价重点实验室开放课题（KFKT2022-8）；广西科技重大专项（桂科AA23023035-4）