目的 探讨α-香附酮对急性肝损伤的保护作用，并基于核因子E2相关因子2（Nrf2）/血红素加氧酶-1（HO-1）通路阐明其作用机制。方法 将C57BL/6小鼠分为对照组、模型组、α-香附酮（85 mg/kg）组，每组8只。测定小鼠血清天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）及肝组织中总超氧化物歧化酶（SOD）、丙二醛（MDA）、白细胞介素（IL）-1β、IL-6水平，观察肝组织病理变化；通过免疫荧光及Western blotting检测Nrf2和HO-1蛋白表达。结果 α-香附酮能显著降低肝损伤小鼠血清ALT、AST水平及MDA、IL-1β、IL-6含量显著降低，SOD含量显著升高（P＜0.01）；α-香附酮组小鼠肝脏上述病理形态有较明显改善，肝小叶结构较为清晰完整，胞质间不规则空洞减少，少见明显炎症细胞浸润。与模型组比较，α-香附酮组中的Nrf2和HO-1蛋白表达显著升高（P＜0.05、0.01）。结论 α-香附酮可能通过激活Nrf2/HO-1通路改善肝损伤。

Objective To investigate the protective effect of α-cyperone on acute liver injury, and elucidate its mechanism based on the Nrf2/HO-1 pathway. Methods The C57BL/6 mice were divided into the control group, the model group, and the α-acorone (85 mg/kg) group, with 8 mice in each group. Determine the levels of serum AST, ALT, and SOD, MDA, IL-1β, and IL-6 in liver tissue, and observe the pathological changes of liver tissue. The expression of Nrf2 and HO-1 proteins was detected by immunofluorescence and Western blotting. Results α-Cyperone can significantly reduce the levels of ALT and AST in the serum of mice with liver injury, as well as the contents of MDA, IL-1β, and IL-6, and significantly increase the content of SOD (P < 0.01). The pathological morphology of the liver in the α-cyperone group showed a more obvious improvement. The liver lobule structure was clearer and more complete, the irregular cavities between the cytoplasm were reduced, and obvious inflammatory cell infiltration was rarely observed. Compared with the model group, the protein expressions of Nrf2 and HO-1 in the α-cyperone group were significantly increased (P < 0.05, 0.01). Conclusion α-Cyperone may improve liver damage by activating the Nrf2/HO-1 pathway.

R285.5;R286.5

国家自然科学基金资助项目（82505032）；国家中医药管理局监测统计中心中医药监测统计研究课题（2025JCTJA16）；山东省青年研究课题大学生专项课题（WL-SQD25064）；2024年齐鲁医药学院校级青年创新团队（X2024QCTD02）；中医药标准化能力提升项目-中医药国际标准关键技术攻关(香附)（600038251005）