目的 利用非靶向代谢组学方法探究消痔丸对巴豆油诱导的肛门肿胀类痔模型大鼠的作用机制。方法 建立巴豆油诱导的大鼠肛门肿胀类痔模型，取对照组、模型组、消痔丸给药组血浆，采用UPLC-Q-TOF-MS法建立血浆代谢指纹谱，筛选潜在生物标志物，结合人类代谢组学数据库（HMDB）和京都基因与基因组百科全书数据库（KEGG）富集相关代谢通路并分析消痔丸对生物标记物的干预作用，进而解析其作用机制。结果 共筛选鉴定出35个痔病潜在生物标志物，涉及甘油磷脂代谢、花生四烯酸代谢、自噬、三羧酸循环、精氨酸和脯氨酸代谢等11条代谢通路，消痔丸干预后对其中23个生物标记物有显著调节作用。结论 消痔丸通过调控甘油磷脂代谢、花生四烯酸代谢、自噬、氨基酸代谢、三羧酸循等信号通路，发挥抗炎、维持供能、调控机体稳态的作用。

Objective To explore the mechanism of Xiaozhi Pills on croton oil-induced anal swelling hemorrhoids model rats by non-targeted metabolomics technology. Methods Rats model of anal swelling hemorrhoids induced by croton oil was established. The plasma of control group, model group, and Xiaozhi Pills group was taken, and the plasma metabolic fingerprint spectrum was established by UPLC-Q-TOF-MS method. Combined with HMDB and KEGG, the related metabolic pathways were enriched and the intervention effect of Xiaozhi Pills on biomarkers was analyzed. Results A total of 35 potential biomarkers of hemorrhoids were screened and identified, involving 11 metabolic pathways such as glycerophospholipid metabolism, arachidonic acid metabolism, autophagy, tricarboxylic acid cycle, arginine and proline metabolism, etc. After the intervention of Xiaozhi Pills, 23 biomarkers were significantly regulated. Conclusion Xiaozhi Pills exerts anti-inflammatory, energy maintenance, and homeostasis regulation effects by regulating signaling pathways such as glycerophospholipid metabolism, arachidonic acid metabolism, autophagy, amino acid metabolism, and the tricarboxylic acid cycle.

R285.5

国有资本经营预算支持省属企业科研项目（2024GZ031）