目的 建立水红花子中多指标成分定量，结合化学计量学评价不同产地水红花子的质量。方法 采用HPLC法测定水红花子中原儿茶酸、儿茶素、异荭草素、荭草素、花旗松素、木犀草素、槲皮素、山柰酚、圣草酚、白桦脂酸和β-谷甾醇，并检测醇溶性浸出物、总灰分和酸不溶性灰分，采用主成分分析、因子分析、正交偏最小二乘法-判别分析、熵权-TOPSIS对检测结果分析。结果 18批水红花子样品自动聚为3类，呈现地域性分布。儿茶素、花旗松素、原儿茶酸、槲皮素、山柰酚、β-谷甾醇和异荭草素7个成分可作为引起不同产地水红花子之间质量差异的主要变量，是质量差异标志物。黑龙江、辽宁和吉林产地的水红花子整体质量较好，其中辽宁西丰县的样品质量最优，广西和四川产的水红花子质量较差。结论 所建立的多组分定量和化学计量学控制模式简单、易推广，可用于水红花子的内在质量评价。

Objective To establish an HPLC multi-index component quantification method for Polygoni Orientalis Fructus, and to evaluate the quality of Polygoni Orientalis Fructus from different areas by chemometrics. Methods Simultaneous determination of protocatechuic acid, catechin, isoorientin, orientin, taxifolin, luteolin, quercetin, kaempferol, eriodictyol, betulinic acid, and β- sitosterol in Polygoni Orientalis Fructus were carried on by HPLC method. Alcohol-soluble extractive, total ash, and acid-insoluble ash were also detected. PCA, FA, OPLS-DA, and EW-TOPSIS methods were used to analyze the results. Results 18 Batches of Polygoni Orientalis Fructus samples were automatically clustered into 3 categories, showing a regional distribution. Seven components, including catechins, taxifolin, protocatechuic acid, quercetin, kaempferol, β-sitosterol, and isoorientin, could serve as the main variables causing quality differences in Polygoni Orientalis Fructus from different areas and were indicators of quality differences. The overall quality of samples from Heilongjiang, Liaoning, and Jilin Province production areas were good, with the sample quality from Xifeng County, Liaoning Province being the best, while the samples from Guangxi and Sichuan Province had poor quality. Conclusion The multi-index component quantification method and chemometric control mode established is simple and easy to generalize, and can be used for the internal quality evaluation for Polygoni Orientalis Fructus.

R286.02

河南省高等学校重点科研指导计划项目（20B550007）