目的 建立猫爪草中成分的HPLC法，利用化学计量学、加权优劣解距离法、灰色关联度融合模型评价不同产地猫爪草质量。方法 建立HPLC外标法测定猫爪草中没食子酸、咖啡酸、鞣花酸、3,4-二甲氧基苯甲酸、原白头翁素、小毛茛内酯、穗花杉双黄酮、7-O-甲基圣草酚、白头翁素、豆甾醇、β-谷甾醇，并检测浸出物、总灰分和酸不溶性灰分，结合化学计量学、加权优劣解距离法与灰色关联度融合模型对猫爪草质量进行综合评价。结果 猫爪草中活性成分批次间质量分数差异较大。18批猫爪草样品被分为3个类群，对猫爪草分类和质量贡献较大的成分有小毛茛内酯、穗花杉双黄酮、没食子酸、咖啡酸和β-谷甾醇。不同产地猫爪草样品质量存在显著差异，河南、安徽和江苏省产猫爪草的质量较优。结论 所建方法简单、科学，为猫爪草的质量控制提供参考。

Objective To establish an HPLC method for components in Ranunculi Ternati Radix, and to evaluate the quality of Ranunculi Ternati Radix from different origins by using the chemometrics, weighted TOPSIS, and grey correlation degree fusion model. Method The contents of gallic acid, caffeic acid, ellagic acid, 3,4-dimethoxybenzoic acid, protoanemonin, ternatolide,amentoflavone, 7-O-methyleriodictyol, anemonine, stigmasterol, and β-sitosterol were determined by HPLC-external standard method. The extract, total ash, and acid-insoluble ash were detected. The quality of Ranunculi Ternati Radix was analyzed by chemometrics, weighted TOPSIS, and grey correlation degree fusion model. Results There were significant differences of active ingredients contents in Ranunculi Ternati Radix between different batches. The 18 batches of Ranunculi Ternati Radix samples were divided into three groups, and the components that contributed significantly to the classification and quality of Ranunculi Ternati Radix included ternatolide, 7-amentoflavone, gallic acid, caffeic acid, and β-sitosterol. There were significant differences in the quality of Ranunculi Ternati Radix samples from different origins, with Henan, Anhui, and Jiangsu Provinces producing better quality Ranunculi Ternati Radix. Conclusion The established method is simple and scientific, providing reference for the quality control of Ranunculi Ternati Radix.

R286.02

河南省高等学校重点科研指导计划项目(20B460010)